Why We Chose the University of Adelaide
When it came time to prove our formula, we didn’t look for the easiest path—we chose the most credible one. We partnered with The University of Adelaide, now called Adelaide University, because we wanted independent, globally respected validation from one of Australia’s leading research institutions. We believe so strongly in our product that we invested over three years, significant funding, and committed to full, peer-reviewed clinical trials on the finished formula—not just individual ingredients.
We completed two independent clinical trials evaluating the finished product at the doses dogs actually receive, with both studies published in peer-reviewed veterinary journals. That level of scrutiny matters. It means our results aren’t marketing claims. They’re scientifically tested, transparently measured, and credible worldwide.
Clinical Trial One – Skin Microbiome Modulation
Published in Veterinary Dermatology (2025)
This study evaluated the effect of daily oral probiotic and postbiotic supplementation on the canine skin microbiome over 90 days.
Using long-read PacBio sequencing, researchers identified species-level microbial changes — not just genus-level trends.
The Results Were Clear
After 90 days:
- 7-fold increase in Lactobacillus acidophilus (FDR p=0.02)
- 40-fold increase in Roseomonas vastitus (FDR p=0.04)
- 37-fold increase in Dubosiella newyorkensis (FDR p=0.02)
- Significant reduction in Staphylococcus pseudintermedius (p=0.02 via culture)
- Overall reduction in Staphylococcus abundance
This was the first study to demonstrate that oral Tri-Biotic supplementation can systemically alter the canine skin microbiota at species level. This is not marketing. This is published data.
Clinical Trial Two – Gut & Skin Axis Study
Published in Veterinary Research Communications (2026)
The second trial expanded the analysis to include fecal microbiota, confirming systemic microbiome coordination.
By Day 90:
- Significant shift in gut microbiome composition (PERMANOVA p=0.05)
- Increased abundance of Lactobacillus acidophilus
- Increased abundance of Lactobacillus johnsonii
- Increased abundance of Limosilactobacillus reuteri
Most importantly:
There was a statistically significant positive correlation between gut and skin microbiota changes.
The gut–skin axis in dogs is not theoretical.
It was measured.
The Tri-Biotic Difference
Most veterinary supplements contain Probiotics alone.
Activ Daily uses a Tri-Biotic formulation:
- Prebiotics to support the growth of beneficial bacteria
- Probiotics containing targeted canine-specific strains
- Postbiotics providing bioactive compounds that support immune modulation and gut barrier integrity
This combined approach is designed to address dysbiosis at a microbiome level rather than providing short-term symptomatic support.
Incorporating Tri-Biotics increases manufacturing costs up to tenfold compared with standard probiotic-only products. We chose this approach deliberately, prioritising biological efficacy and clinical outcomes over cost minimisation.
Both clinical trials utilised PacBio long-read full-length 16S rRNA gene sequencing, enabling:
- Species-level microbial identification
- Accurate differentiation of Staphylococcus species
- Detection of thousands of bacterial taxa
- Comprehensive microbiome composition analysis
This methodology provides a higher-resolution assessment of microbiome shifts compared with standard short-read sequencing techniques.
Strain Specificity Matters
Not all probiotics demonstrate equivalent biological activity.
Even within the same bacterial species, strain-level variation significantly influences functional outcomes.
Our proprietary ADH™ strains were selected through a structured research and development process and subsequently evaluated in controlled canine clinical trials.
Human-derived probiotic strains are not designed to align with canine-specific microbiome composition or host–microbe interactions.
Generic multi-strain blends typically do not account for species-specific microbial ecology.
Microbiome Assist™ is our strain-selection and formulation platform designed to ensure the selected strains are tailored specifically to canine microbiome requirements.
Clinical Relevance for Veterinary Practice
Activ Dog Health supplements are formulated to provide microbiome support in cases such as:
- Post-antibiotic recovery
- Chronic dermatological presentations
- Recurrent gastrointestinal disturbance
- Immune-mediated imbalance
- Behavioural presentations where gut–brain axis involvement is suspected
These products are positioned as microbiome-supportive adjuncts, not pharmaceutical substitutes.
The objective is to provide a science-backed nutritional strategy that supports restoration of microbial balance and long-term gastrointestinal resilience within a broader clinical management plan.
The Benchmark Has Been Set
Clinical Relevance for Veterinary Practice
The data supports Activ Daily as a microbiome-modulating adjunct for:
- Post-antibiotic recovery
- Dysbiosis-associated dermatological cases
- Chronic skin management protocols
- Immune-modulated inflammatory conditions
- Long-term microbiome resilience strategies
This provides veterinarians with a non-antimicrobial intervention supported by published evidence.
In an era of antimicrobial resistance, this matters.
Scientific Integrity
- University ethics approved
- Peer-reviewed publications
- Controlled longitudinal design
- Long-read sequencing methodology
- Transparent statistical analysis (FDR correction, PERMANOVA, GLM modelling)
We invested years into research because credibility cannot be fabricated.
It must be earned.
Access the Research
Veterinary professionals and qualified practitioners may request:
- Clinical trial summaries
- Study methodology details
- Published peer-reviewed paper
For professional enquiries:
info@activdog.com
