Why We Chose Adelaide University
As veterinarians know, much of the animal supplement industry is built on ingredient claims, not finished-product proof.
Small amounts of ingredients are often added to formulas, listed on labels, and marketed with benefits that have never been tested in the final product.
We chose a different path.
We brought together leading veterinarians, microbiologists and research scientists to develop Activ Daily ULTIMATE. Then, when it came time to validate the formula, we did not rely on in-house testing, ingredient studies or theoretical claims.
We partnered with Adelaide University and invested more than three years and significant funding into clinical trials on the finished formula, at the same dose dogs actually receive.
The result: two completed clinical trials, both published in peer-reviewed veterinary journals.
Not ingredient research.
Not marketing claims.
Not theoretical benefits.
Finished-formula evidence on the final product.
That level of validation is rare in the canine supplement industry. It means Activ Daily ULTIMATE has been scientifically tested, independently reviewed and held to the standard expected of credible veterinary research.
While others make claims, we chose to test ours.
Clinical Trial One – Skin Microbiome Modulation
Published in Veterinary Dermatology (2025)
This study evaluated the effect of daily oral Tri-Biotic supplementation on the canine skin microbiome over 90 days.
Using long-read PacBio sequencing, researchers identified species-level microbial changes, not just genus-level trends.
Key Findings After 90 Days
After 90 days:
- 7-fold increase in Lactobacillus acidophilus (FDR p=0.02)
- 40-fold increase in Roseomonas vastitus (FDR p=0.04)
- 37-fold increase in Dubosiella newyorkensis (FDR p=0.02)
- Significant reduction in Staphylococcus pseudintermedius (p=0.02 via culture)
- Overall reduction in Staphylococcus abundance
The study showed that daily oral Tri-Biotic supplementation was associated with measurable changes in the canine skin microbiota at species level. This is published data, not ingredient-level marketing.
Clinical Trial Two – Gut & Skin Axis Study
Published in Veterinary Research Communications (2026)
The second trial expanded the analysis to include faecal microbiota, investigating the relationship between gut and skin microbiome changes over 90 days.
By Day 90:
- Significant shift in gut microbiome composition (PERMANOVA p=0.05)
- Increased abundance of Lactobacillus acidophilus
- Increased abundance of Lactobacillus johnsonii
- Increased abundance of Limosilactobacillus reuteri
Most importantly:
A statistically significant positive correlation was observed between gut and skin microbiota changes.
The gut–skin axis in dogs is not theoretical.
It was measured.
The Tri-Biotic Difference
Most veterinary supplements contain Probiotics alone.
Activ Daily ULTIMATE uses a complete Tri-Biotic formulation:
- Prebiotics to support the growth of beneficial bacteria
- Probiotics containing targeted canine-specific strains
- Postbiotics providing bioactive compounds that support the gut environment, immune response and gut barrier function
This combined approach is designed to support microbiome balance at a deeper level rather than focusing only on short-term symptom support.
Incorporating Tri-Biotics increases manufacturing costs compared with standard probiotic-only products. We chose this approach deliberately because meaningful microbiome support depends on the right ingredients, in the right balance, delivered in the right format.
Both clinical trials utilised PacBio long-read full-length 16S rRNA gene sequencing, enabling:
- Species-level microbial identification
- Accurate differentiation of Staphylococcus species
- Detection of thousands of bacterial taxa
- Comprehensive microbiome composition analysis
This methodology provides a higher-resolution assessment of microbiome shifts compared with standard short-read sequencing techniques.
Strain Specificity Matters
Not all probiotics demonstrate equivalent biological activity.
Even within the same bacterial species, strain-level variation significantly influences functional outcomes.
Our proprietary ADH™ strains were selected through a structured research and development process and subsequently evaluated in controlled canine clinical trials.
Human-derived probiotic strains are not designed around canine microbiome composition or host–microbe interactions.
Generic multi-strain blends often fail to account for species-specific microbial ecology.
Microbiome Assist™ is our strain-selection and formulation platform designed to ensure the selected strains are tailored specifically to canine microbiome requirements.
Clinical Relevance for Veterinary Practice
Activ Pet Health supplements are formulated to provide microbiome support in cases such as:
- Post-antibiotic recovery
- Chronic dermatological presentations
- Recurrent gastrointestinal disturbance
- Immune-mediated imbalance
- Behavioural presentations where gut–brain axis involvement is suspected
These products are positioned as microbiome-supportive adjuncts, not pharmaceutical substitutes.
The objective is to provide a science-backed nutritional strategy that supports microbial balance and long-term gastrointestinal resilience within a broader clinical management plan.
The Benchmark Has Been Set
Where Activ Daily ULTIMATE Fits in Veterinary Practice
The data supports Activ Daily ULTIMATE as a microbiome-modulating adjunct for:
- Post-antibiotic recovery
- Dermatological cases where microbiome imbalance may be relevant
- Recurrent gastrointestinal disturbance
- Immune and inflammatory balance support
- Behavioural presentations where gut–brain axis support may be considered
This provides veterinarians with a non-antimicrobial intervention supported by published evidence.
In an era of antimicrobial resistance, this matters.
Scientific Integrity
- University ethics approved
- Peer-reviewed publications
- Controlled longitudinal design
- Long-read sequencing methodology
- Transparent statistical analysis (FDR correction, PERMANOVA, GLM modelling)
We invested years into research because credibility cannot be fabricated.
It must be earned.
Access the Research
Veterinary professionals and qualified practitioners may request:
- Clinical trial summaries
- Study methodology details
- Published peer-reviewed paper
For professional enquiries:
info@activdog.com
